Directing Biosynthesis VI - Book of abstracts

Directing Biosynthesis VI - Book of abstracts

27 - 29 June 2022, Edinburgh, UK Directing Biosynthesis VI #DBVI

Book of Abstracts

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Welcome

Dear Colleague A very warm welcome to Directing Biosynthesis VI. On behalf of the Scientific Committee, I’d like to thank you all for taking part in what I am sure will be an exceptionally stimulating and enjoyable three days! As we all know, specialised metabolites produced by microorganisms and plants have inspired the development of many blockbuster drugs, crop protection agents and other high value chemicals. Research on the biosynthesis of such metabolites is not only of strategic importance commercially - it is also relevant to wider society and continues to play a key role in addressing emerging global challenges in health, the environment and sustainability. This year’s Directing Biosynthesis conference is the sixth in the series. The first conference was held in Cambridge in 2006 and followed on from earlier meetings focused more specifically on polyketide biosynthesis. We originally planned to hold this sixth edition in June 2020 but were forced to postpone it due to the COVID pandemic. In the interim, we held a two-day online meeting (in April 2021), which proved to be an excellent forum for disseminating the latest advances in the field despite the difficult circumstances most of us were enduring at that time. Modern biosynthesis research is exceptionally interdisciplinary, employing a wide range of techniques from diverse fields, including (meta)genomics, molecular genetics, enzymology, structural biology, ecology, evolutionary biology, organic synthesis, analytical chemistry, and synthetic biology. The ongoing success of this conference series serves to highlight the impact of our efforts as a community in this exciting field of contemporary science. Doubtless this year’s meeting will provide ample opportunity for attendees to discuss their work and initiate or renew productive collaborations. I hope you enjoy the mix of presentations, not only the keynote and contributed lectures, but also the wide range of posters and I really hope that this meeting will provide an opportunity for early career researchers to share their ideas and enthusiasm. I would like to extend thanks to all the sponsors, supporters and co-organizers who have promoted and supported this event. I strongly encourage all participants to take the opportunity to meet with members of these organizations over the next three days and learn about how we can work together to continue advancing this very exciting field. Finally, I am particularly grateful to colleagues both on the Scientific Committee and at the Royal Society of Chemistry (RSC) for their many and varied contributions to making this conference happen. Greg Challis Chair of Scientific Committee University of Warwick / Monash University

Meeting Information

Directing Biosynthesis VI is hosted by the Chemistry Biology Interface Division (CBID) of the Royal Society of Chemistry. www.rsc.org/CBID This book contains abstracts of the oral and poster presentations to be presented at the conference. All abstracts in this book are reproduced directly from typescripts supplied by the authors. Copyright remains with the authors.

Lectures All lectures will take place in the Pentland Suite, John McIntyre Conference Centre (1st Floor).

Catering Please refer to programme for catering locations.

Conference Dinner The dinner will be held on the evening of Tuesday 28 June and will take place at the Royal Botanic Garden Edinburgh from 19.00 Transport will be provided, please check at the registration desk for times. The cost of the dinner is included in the registration fee for in person delegates, if you are no longer able to attend please let us know.

Posters These will be on display throughout the conference in South Hall, a short walk from the main conference centre

Posters have been numbered consecutively: P01-P99 The poster sessions will take place in South Hall Monday June 27 , 15:10 – 16:20 (even numbers) and 18:00 – 19:00 (all posters) Tuesday June 28 , 13:00 – 15:00 (odd numbers)

The posters will be available to view throughout the discussion by clicking on the link in the virtual lobby. If you are an online poster presenter, please ensure that you are logged into the poster room assigned to your poster number in the lobby. Posters with a F will be presented during the flash poster presentations, taking place before the poster sessions.

Student Poster Prizes

Kindly sponsored by:

Sponsors and Exhibitors

Exhibitor

Isomerase is one of the UK’s leading biotechnology service providers and has been supporting partners for over 10 years. We combine cutting-edge synthetic biology, advanced strain bioengineering, process development and biosynthetic chemistry to create highly productive strains, and efficient cost-effective routes to commercially attractive products. For customers working with microbial products and processes in a wide variety of sectors, Isomerase offers flexible and agile biotechnology services for those partners looking for more than just a CRO.

Sponsor

NCIMB curates a unique collection of microorganisms with a host of potential industrial applications, as well as providing laboratory services, microbial identification, whole genome sequencing, microbial community analysis, qPCR and storage services. We also work collaboratively with other organisations to unlock value from microorganisms.

Scientific Committee

Professor Greg Challis (Chair) University of Warwick, UK and Monash University, Australia

Professor Sarah Barry Kings College London, UK

Professor Dominic Campopiano University of Edinburgh, UK

Professor Hai Deng University of Aberdeen, UK

Professor Kira Weissman University of Lorraine, France

Speakers

Ikuro Abe, Ph. D. Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan Ikuro Abe is Professor of Natural Products Chemistry at Graduate School of Pharmaceutical Sciences, The University of Tokyo (2009-). He received his B.S. (1984) and Ph.D. (1989) from The University of Tokyo. After two years postdoctoral research with Professor Guy Ourisson at the CNRS Institut de Chimie des Substances Naturelles, and mostly with Professor Michel Rohmer at the Ecole Nationale Supérieure de Chimie de Mulhouse in France (1989-1991), he moved to the USA to work with Professor Glenn D. Prestwich at the State University of New York at Stony Brook (1991-1996) and then at The University of Utah (1996-1998). His research interests mostly focus on exploring and engineering the natural products biosynthesis. He has authored 200+ publications including Nature, Nat. Chem. Biol., Nat. Commun., JACS, ACIE, and PNAS. He received the Pharmaceutical Society of Japan Award in 2019, and Prizes for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology, Japan. He is a former President of The Japanese Society of Pharmacognosy.

Squire J. Booker Pennsylvania State University, USA

Squire J. Booker is an Evan Pugh Professor of Chemistry and of Biochemistry and Molecular Biology, and the Eberly Family Distinguished Chair in the College of Science at the Pennsylvania State University. He is also an investigator of the Howard Hughes Medical Institute. He received a B.A. degree with a concentration in chemistry from Austin College in 1987 and a Ph.D. in biochemistry from the Massachusetts Institute of Technology in 1994. After postdoctoral studies in Paris, France and at the University of Wisconsin, he joined the faculty at Penn State in 1999. Booker’s research focuses on natural product biosynthesis, antibiotic resistance and metalloenzymology, with a particular emphasis on the methylation and sulfhydrylation of unactivated carbon centers.

David Craik, FAA, FRS The University of Queensland, Brisbane, Australia

David Craik, FAA, FRS, is a group leader and Professor of Chemistry at the Institute for Molecular Bioscience at The University of Queensland, Brisbane, Australia. He obtained his PhD in organic chemistry from La Trobe University in Melbourne, Australia and undertook postdoctoral studies at Florida State and Syracuse Universities before taking up a lectureship at the Victorian College of Pharmacy in 1983. He was appointed Professor of Medicinal Chemistry and Head of School in 1988. He moved to University of Queensland in 1995 to set up a new biomolecular NMR laboratory and is currently the Director of the Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science. His research focuses on applications of cyclic peptides, toxins and NMR in drug design. He is a Fellow of the Royal Society and a Fellow of the Australian Academy of Science, is author of more than 750 papers and has trained 70 PhD students.

Dr. Ania Fryszkowska Director of Biocatalysis in Process R&D Enabling Technologies, USA

Dr. Ania Fryszkowska the Director of Biocatalysis in Process R&D Enabling Technologies at Merck Sharp and Dohme, USA, where she is responsible for driving the application of enzymes to support the drug pipeline from discovery to commercial launch. Ania received her Ph. D. in organic synthesis from the Warsaw University of Technology and then went on to complete post-doctoral studies with Professor Nigel Scrutton at the University of Manchester working on flavin-dependent enzymes. After five successful years at Dr. Reddy’s Laboratories in the UK, she joined the Biocatalysis group at Merck in 2015, where she has played a pivotal role in driving innovation and expanding biocatalytic tools for process and medicinal chemistry. Ania co-led the development of the novel nine-enzyme cascade process for islatravir (Science, 2019), focusing on the sugar portion. She was a key contributor to inventing the suite of enzymatic methods for site-selective protein functionalization, utilized in the syntheses of insulin bioconjugates MK-5160 and MK-1029 (Science, just accepted). She authored over 25 publications and patents and her scientific contributions were recognized by many awards, including the ACS Young Investigator Award in 2020. Ania co-organized a series of Virtual Biocatalysis and Protein Engineering Meet-ups, which served as a platform for early-career scientists from industry and academia to share their science during the pandemic. Christian Hertweck Leibniz Institute for Natural Product Research and Infection Biology (HKI), Germany Christian Hertweck is the Head of Department Biomolecular Chemistry and Deputy Director at the Leibniz Institute for Natural Product Research and Infection Biology (HKI), and a Full Professor in the Faculty of Biological Sciences at the Friedrich Schiller University Jena. His research focuses on the discovery of microbial natural products, elucidating and harnessing their biosynthesis (genome mining, pathway engineering), and studying the molecular basis of microbial interactions. In recognition of his group’s contribution to the field he was granted numerous awards including the Gottfried Wilhelm Leibniz Prize, and was elected as member of the German National Academy (Leopoldina) in 2015.

Timm Maier University of Basel, Switzerland

Timm Maier studied Biochemistry at the University of Tübingen, Germany, and completed his Doctorate in Structural Biology with Wolfram Saenger at Freie Universität, Berlin, Germany, in 2003. Timm Maier then moved as a Postdoc to the lab of Nenad Ban at ETH Zurich, Switzerland, where he was promoted to a team leader and lecturer position in 2006. In 2011, Timm Maier moved to the Biozentrum of the University of Basel as tenure track Assistant Professor and is Associate Professor of Structural Biology at Biozentrum since 2016. He and his team are best known for structural studies on giant multienzymes, in particular fatty acid and polyketide synthases, as well as on metabolic regulation and mTOR complexes.

Martin Schmeing Yale University, USA

Martin performed graduate research with Tom Steitz at Yale University, studying the architecture and mechanism of the large ribosomal subunit. He then performed postdoctoral training at the LMB, Cambridge, with Venki Ramakrishnan, using cryo- EM and X-ray crystallography to investigate initiation and elongation of translation. Martin established his own laboratory at McGill University in 2010, where he studies nonribosomal peptide synthetases (NRPSs). Two aspects of particular focus of the group’s NRPS research are the catalytic event which links substrate building blocks into peptide products, and the manner in which these enzymes’ domains and modules work together in a complicated and productive catalytic cycle. Martin is currently an Associate Professor of the Department of Biochemistry, the Director of the McGill Centre for Structural Biology, an Associate Director of the McGill Facility for EM Research and the Canada Research Chair in Macromolecular Machines.

Ren Xiang Tan Nanjing University, China

China Pharmaceutical Univ. (BS 1983, MS 1986), Lanzhou Univ. (Ph D 1990, Prof. Z. J. Jia), Technical Univ. Berlin (Visiting Ph D candidate, 1989-1990, Prof. F. Bohlmann), Univ. Lausanne (Visiting scholar, 1995 and 1997, Prof. K. Hostettmann), Univ. California San Diego (Visiting scholar, 2001 and 2003, Prof. W. Fenical), Nanjing Univ. (Associate Prof., 1992; Prof., 1994-present), Nanjing Univ. of Chinese Medicine (Chair Prof., 2016-present; Vice-president, 2016-2018). He works on the discovery and biosynthesis of symbiont- derived bioactive natural products. Yi Tang Penn State University, USA Yi Tang received his undergraduate degree in Chemical Engineering and Material Science from Penn State University. He received his Ph.D. in Chemical Engineering from California Institute of Technology under the guidance of Prof. David A. Tirrell. After NIH postdoctoral training in Chemical Biology from Prof. Chaitan Khosla at Stanford University, he started his independent career at University of California Los Angeles in 2004. He is currently a professor at the Department of Chemical and Biomolecular Engineering at UCLA, and holds joint appointments in the Department of Chemistry and Biochemistry; and Department of Bioengineering. His awards include the ACS Arthur C. Cope Scholar Award (2012), the EPA Presidential Green Chemistry Challenge Award (2012), NIH DP1 Director Pioneer Award (2012) and the ACS Eli Lilly Award in Biological Chemistry (2014)

Professor Chris Willis University of Bristol, UK

Chris Willis is currently Head of the Organic and Biological Chemistry Section at the University of Bristol. Her collaborative research programmes focus on the use of both synthetic biology and organic synthesis to elucidate and manipulate biosynthetic pathways to deliver novel bioactive compounds and biocatalysts, leading to >170 publications. She was awarded the RSC Flintoff Medal in 2008, was a member of the Bristol Polyketides Group awarded the 2013 Rita and John Cornforth Award and recently was recognised by an IUPAC 2019 Distinguished Women in Chemistry or Chemical Engineering Award.

Wenjun Zhang University of California, USA

Wenjun Zhang is an Associate Professor in the Department of Chemical and Biomolecular Engineering at University of California Berkeley and the Charles R. Wilke Endowed Chair in Chemical Engineering. She did her doctoral training with Yi Tang at UCLA and her postdoctoral training with Christopher T. Walsh at Harvard Medical School before joining UC Berkeley in 2011. She is the author of 70+ scientific publications and received awards such as Pew Scholar (2012), NIH Director’s New Innovator (2015), Sloan Research Fellow (2016), American Cancer Society Research Scholar (2017), Presidential Early Career Award for Scientists and Engineers (2019), etc. Zhang Lab is broadly interested in natural product discovery, biosynthesis, engineering, and biological studies.

Programme

Monday 27 June 2022 (all timings are BST)

Registration and refreshments (Lunch available from 12:00) JMCC

11:00

Welcome and introduction Greg Challis University of Warwick

12:50

Session 1: Fungal natural products Session chair: Kira Weissman

Genome mining for unknown unknowns in fungi Yi Tang UC Los Angeles, USA

13:00

K01

Structural studies of transient and higher-order interactions in polyketide synthases Timm Maier University of Basel, Switzerland Identification and characterization of bifunctional fungal terpene synthases Jaclyn Winter University of Utah, USA

13:40

K02

14:20

C01

Flash poster presentations (even numbers)

14:40

Poster session (even numbers) and refreshments South Hall

15:10

Session 2: Peptide Biosynthesis Session chair: Hajo Kries

Structures and functions of nonribosomal and non-nonribosomal peptide synthetases Martin Schmeing McGill University, Canada Structure and function of a peptide crosslinking P450 from biarylitide biosynthesis Max Cryle Monash University, Australia

16:20

K03

17:00

C02

Iterative peptide backbone N-methylation in borosin RiPP biosynthesis Michael Freeman University of Minnesota, USA

17:20

C03

17:40 Investigating the mechanisms of lanthipeptide biosynthesis using structural mass spectrometry Chris Thibodeaux McGill University, Canada C04

Poster session (all posters) and wine reception South Hall

18:00

19:00

Close of sessions

Programme

Tuesday 28 June 2022 (all timings are BST)

Session 3: Biosynthetic engineering I Session chair: Sacha Pidot

Repurposed biocatalysts for the assembly of antibiotics and toxins Christian Hertweck Leibniz Institute for Natural Product Research and Infection Biology (HKI), Germany Engineering of polyketide synthase leads to ‘low-fat’ stambomycins Su Li** Max-Planck-Institute for Terrestrial Microbiology, Germany

09.00

K04

09.40

C05

10.00 CRISPR-Cas9 in vivo editing enables rapid engineering of a complex antibiotic assembly line Wei Li Thong University of Manchester, UK

C06

Towards new more efficient and clinically relevant lincosamide antibiotics Zdenek Kamenik Institute of Microbiology Czech Acad Sci, Czech Republic

10.20

C07

Refreshments South Hall Kindly sponsored by:

10.40

Session 4: Unusual C-C bond forming enzymes Session chair: Sarah Barry

New insights into cobalamin-dependent methyltransferase reactions Squire Booker** Pennsylvania State University, USA Biosynthesis of the unusual carbon skeleton of nocuolin A T eresa Patricia Martins CIIMAR, Portugal

11.10

K05

11.50

C08

12.10 Enzymatic assembly of the salinosporamide gamma-lactam-beta-lactone anticancer warhead Katherine Bauman University of San Diego, USA

C09

Flash poster presentations (odd numbers)

12.30

** presenting online

Programme

Tuesday 28 June 2022 (all timings are BST)

Poster session from 14:00 (odd numbers) and lunch Session to include any online-only posters in addition to in-person South Hall

13:00

Session 5: Polyketide biosynthesis in Gram-negative bacteria Session chair: Jeroen Dickschat

Combining organic synthesis and synthetic biology to explore and exploit polyketide biosynthesis Chris Willis University of Bristol, UK Unveiling the iterative programming of the zeamine II enzymatic assembly line Sophie Dekimpe KU Leuven, Belgium

15.00

K06

15:40

C10

Modular α-hydroxylation in trans-AT PKSs Franziska Hemmerling ETH Zürich, Switzerland

16:00

C11

Refreshments South Hall

16.20

Session 6: Plant natural products Session chair: Stefan Schulz

16:50 Biosynthetic pathways of cyclotides- ultrastable cyclic peptides from plants with applications in agriculture and medicine David Craik University of Queensland, Australia K07

Deciphering quassinoid biosynthesis in the invasive tree of heaven (Ailanthus altissima) Jakob Franke Leibniz University Hannover, Germany Omics approaches to harness daphniphyllum alkaloid diversity Benjamin Lichman University of York, UK

17:30

C13

17:50

C14

18:10

Close of sessions

18:30

Coaches leave for Conference Dinner

19:00 Conference dinner – Royal Botanic Garden Edinburgh

Programme

Wednesday 29 June 2022 (all timings are BST) Session 7: Discovery and biosynthesis of nitrogenous metabolites Session chair: Hai Deng

NAD as a building block in natural product biosynthesis Ikuro Abe University of Tokyo, Japan

9:00

K08

TBC Ren Xiang Tan** Nanjing University, China

9:40

K09

Exploring new biocatalysts for the sphingolipid synthesis in actinobacteria Gustavo Perez-Ortiz The University of Edinburgh, UK Pseudomonas pan-genomic analysis informs the discovery of plant pathogen inhibitors Andrew Truman John Innes Centre, UK

10:20

C15

10:40

C16

11:00 Refreshments South Hall Session 8: Biosynthetic engineering II Session chair: Dominic Campopiano

Enzymatic routes to the next generation therapeutics Anna Fryszkowska Merck Sharp & Dohme, USA

11:30

K10

Simulation-guided redesign of terpene synthase product outcome Marc Van der Kamp University of Bristol, UK

C17

12:10

Enzymatic methylation in engineered biosynthetic pathways as a tool for product diversification Kristina Haslinger Rijksuniversiteit Groningen, Netherlands Fatty acid synthases (FASs) enable access to new-to-nature compounds Martin Grininger Goethe University Frankfurt, Germany

12:30

C18

12.50

C19

Lunch South Hall

13.10

Round table discussions for early-career researchers Informal discussions over lunch focusing on career directions, different systems, challenges and skills, with advice from scientists in academia and industry

13:40

Session 9: New frontiers Session chair: Greg Challis

Biosynthesis of unusual functionalities in natural products Wenjun Zhang UC Berkeley, USA Closing remarks and presentation of poster prizes Greg Challis, University of Warwick Kindly sponsored by:

14:40

K11

15.20

15.30 Close - refreshments available - JMCC

** presenting online

Keynote presentations

K01

Genome mining for unknown unknowns in fungi Yi Tang University of California, Los Angeles, USA

K02

Structural studies of transient and higher-order interactions in polyketide synthases Timm Maier Biozentrum, University of Basel, Switzerland

K03

Structures and functions of nonribosomal and non-nonribosomal peptide synthetases

Martin Schmeing McGill, Canada

K04

Repurposed biocatalysts for the assembly of antibiotics and toxins Christian Hertweck Leibniz Institute for Natural Product Research and Infection Biology (HKI), Germany New insights into cobalamin-dependent methyltransferase reactions Squire Booker Penn State University, USA Combining organic synthesis and synthetic biology to explore and exploit polyketide biosynthesis Chris Willis University of Bristol, UK Biosynthetic pathways of cyclotides- ultrastable cyclic peptides from plants with applications in agriculture and medicine Daivd Craik University of Queensland, Australia

K05

K06

K07

K08

NAD as a building block in natural product biosynthesis Ikuro Abe The University of Tokyo, Japan

K09

TBC Ren Xiang Tan Nanjing University, China

K10

Enzymatic routes to the next generation therapeutics Ania Fryszkowska Merck Sharp & Dohme, USA Biosynthesis of unusual functionalities in natural products Wenjun Zhang UC Berkeley, USA

K11

Oral presentations

C01

Identification and characterization of bifunctional fungal terpene synthases Jaclyn Winter University of Utah, USA Structure and function of a peptide crosslinking P450 from biarylitide biosynthesis Max Cryle Monash University, Australia Iterative peptide backbone N-methylation in borosin RiPP biosynthesis Michael Freeman University of Minnesota, USA

C02

C03

C04

Investigating the mechanisms of lanthipeptide biosynthesis using structural mass spectrometry

Christopher Thibodeaux McGill University, Canada

C05

Engineering of polyketide synthase leads to ‘low-fat’ stambomycins Li Su Max-Planck-Institute for Terrestrial Microbiology, Germany CRISPR-Cas9 in vivo editing enables rapid engineering of a complex antibiotic assembly line Wei Li Thong University of Manchester, UK Towards new more efficient and clinically relevant lincosamide antibiotics Zdenek Kamenik Institute of Microbiology Czech Acad Sci, Czech Republic Biosynthesis of the unusual carbon skeleton of nocuolin A Teresa Patricia Martins ICBAS, Portugal Enzymatic assembly of the salinosporamide gamma-lactam-beta-lactone anticancer warhead Katherine Bauman The Regents of the Univ. of Calif., USA

C06

C07

C08

C09

C10

Unveiling the iterative programming of the zeamine II enzymatic assembly line Sofie Dekimpe KU Leuven, Belgium

C11

Modular α-hydroxylation in trans-AT PKSs Franziska Hemmerling ETH Zürich, Switzerland

C13

Deciphering quassinoid biosynthesis in the invasive tree of heaven (Ailanthus altissima) Jakob Franke Leibniz University Hannover, Germany OMICS approaches to harness daphniphyllum alkaloid diversity Benjamin Lichman University of York, UK Exploring new biocatalysts for the sphingolipid synthesis in Actinobacteria Gustavo Perez Ortiz The University of Edinburgh, UK Pseudomonas pan-genomic analysis informs the discovery of plant pathogen inhibitors Andrew Truman John Innes Centre, UK Simulation-guided redesign of terpene synthase product outcome Marc Van der Kamp University of Bristol, UK Enzymatic methylation in engineered biosynthetic pathways as a tool for product diversification Kristina Haslinger Rijksuniversiteit Groningen, Netherlands Fatty acid synthases (FASs) enable access to new-to-nature compounds Martin Grininger Goethe University Frankfurt, Germany

C14

C15

C16

C17

C18

C19

Poster presentations

P01

Fischerazoles A-C, cyanobacterial polychlorinated lipids featuring fatty acyl chain rearrangement Kathleen Abt CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, Portugal

P02F

The evolution of glycopeptide antibiotics Martina Adamek University of Tuebingen, Germany

P03

Study of the mechanism of action and biosynthesis of microbial secondary metabolites Duha Alkhder The University of Warwick, UK

P04

A versatile spirotetronate cyclase Jawaher Alnawah University of Bristol, UK

P05F

Application of Acyl-S-acetylcysteamine (Acyl-SNAC) thiosters as synthetic mimic in biosynthetic intermediates acyl- CoAs Saad Alrashdi University of Aberdeen, UK Producing engineered lipopeptides via the ribosomal route Nina Bösch ETH Zürich, Switzerland Chloro-methoxy-tryptophan biogenesis during NRP biosynthesis Michael Brigham University of Leeds, UK Bacterial endosymbionts protect beneficial soil fungus from nematode attack Hannah Büttner Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Germany

P06

P07F

P08F

P09F

Discovery and heterologous expression of new microviridins from a Portuguese Culture Collection

Raquel Castelo-Branco CIIMAR/FCUP, Portugal

P10

Identification of the biosynthetic gene cluster for the antiviral spirotetronate MM46115, and investigation of a putative diene forming dehydratase domain Rebecca Clayton University of Warwick, UK Discovery of demurilactone A; a novel growth inhibitor of L-form Bacillus Subtilis Yousef Dashti Newcastle University, UK Engineering a bacterial biosynthetic pathway for the synthesis of novel anticancer agents Dries De Ruysscher KU Leuven - VIB Center for Microbiology, Belgium α-Methylenation of metabolic aldehydes via a biocompatible aldol condensation Jonathan Dennis University of Edinburgh, UK Structural basis of the specificity of amidinohydrolases involved in the final steps of the biosyntheses of large linear and cyclic polyketides Marcio DIAS University of Sao Paulo, Brazil Exploring the role of nonribosomal peptides in vaginal communities Jelle Dillen University of Antwerp, Belgium Chemical and enzymatic macrocyclisation of antibiotic peptides Yaoyu Ding King’s College London, UK

P11

P12

P13

P14

P15F

P16F

P17

Illuminating and exploiting programmed O-methylation in trans-AT polyketide synthases James Duncan University of Warwick, UK Exploring the natural diversity of acyltransferases producing gene- encoded lipopeptides. Johannes Eckert ETH Zürich, Switzerland Characterization of the antipathogenic molecules of lactobacilli in vegetable fermentations Tom Eilers Uantwerpen, Belgium Transformation associated recombination (TAR) cloning of promising antibiotic producing biosynthetic gene clusters (BGCs) Fayrouz El Maddah University of Warwick, UK Opening the circle – characterisation of a unique esterase in faulknamycin biosynthesis Asif Fazal University of Leeds, UK Thioesterase-mediated O-acetylation and double bond formation in peloruside biosynthesis Amy Fraley Eidgenössische Technische Hochschule Zürich, Switzerland Engineering microcin J25 for new biological activities and therapeutic applications Hans Gerstmans KU Leuven - VIB, Belgium The genetic basis of biosynthesis and anti-inflammatory activity of calendula officinalis triterpene fatty acid esters Daria Golubova Earlham Institute, UK

P18

P19

P20

P21

P22F

P23

P24F

P25

Investigating bioactive natural products from the fungus Escovopsis weberi Claudio Greco John Innes Centre, UK Adenylation domain substrate selectivity in fungal nonribosomal peptide biosynthesis Stephanie Heard University of Utah, USA Investigation of the release and cyclisation steps from the PKS-NRPS hybrid during the biosynthesis of cytochalasans Henrike Heinemann University of Hanover, Germany Small molecule discovery from LC-MS >Page 1 Page 2 Page 3 Page 4 Page 5 Page 6 Page 7 Page 8 Page 9 Page 10 Page 11 Page 12 Page 13 Page 14 Page 15 Page 16 Page 17 Page 18 Page 19 Page 20 Page 21 Page 22 Page 23 Page 24 Page 25 Page 26 Page 27 Page 28 Page 29 Page 30 Page 31 Page 32 Page 33 Page 34 Page 35 Page 36 Page 37 Page 38 Page 39 Page 40 Page 41 Page 42 Page 43 Page 44 Page 45 Page 46 Page 47 Page 48 Page 49 Page 50 Page 51 Page 52 Page 53 Page 54 Page 55 Page 56 Page 57 Page 58 Page 59 Page 60 Page 61 Page 62 Page 63 Page 64 Page 65 Page 66 Page 67 Page 68 Page 69 Page 70 Page 71 Page 72 Page 73 Page 74 Page 75 Page 76 Page 77 Page 78 Page 79 Page 80 Page 81 Page 82 Page 83 Page 84 Page 85 Page 86 Page 87 Page 88 Page 89 Page 90 Page 91 Page 92 Page 93 Page 94 Page 95 Page 96 Page 97 Page 98 Page 99 Page 100 Page 101 Page 102 Page 103 Page 104 Page 105 Page 106 Page 107 Page 108 Page 109 Page 110 Page 111 Page 112 Page 113 Page 114 Page 115 Page 116 Page 117 Page 118 Page 119 Page 120 Page 121 Page 122 Page 123 Page 124 Page 125 Page 126 Page 127 Page 128 Page 129 Page 130 Page 131 Page 132 Page 133 Page 134 Page 135 Page 136 Page 137 Page 138 Page 139 Page 140 Page 141 Page 142 Page 143 Page 144 Page 145 Page 146 Page 147 Page 148 Page 149 Page 150 Page 151 Page 152 Page 153 Page 154 Page 155 Page 156 Page 157 Page 158 Page 159 Page 160 Page 161 Page 162

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